Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. For details on our patents, please refer to RabMAb ® patents. ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). - High batch-to-batch consistency and reproducibility.This product is a recombinant monoclonal antibody, which offers several advantages including: Maxpar ® is a trademark of Fluidigm Canada Inc. This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. A human DNA fragmentation factor (DFF) that is cleaved by caspase-3 during apoptosis has been identified. Catalog No.: 2424 Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Anti-ICAD (CT) Antibody ORDERING INFORMATION. mass cytometry) and Multiplex Imaging applications. Please use the filters to sort the attributes in the table below in order to find the antibody that fits your exact requirements. Find MSDS or SDS, a COA, data sheets and more information. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. Our range of ICAD antibodies (also known as DFF-45) can be used for applications including western blotting and immunohistochemistry, and are specific for either the C or N terminal region of the protein. Anti-DFF45/35 Antibody, NT This Anti-DFF45/35 Antibody, N-terminus is validated for use in IP, WB for the detection of DFF45/35. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
#Icad antibody free
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. Fractions were collected from the bottom of the tube, and one-quarter aliquots of each fraction and 2 of the input protein (lane 11) were analyzed by Western blotting with anti-HA antibody for CAD (top panel) or anti-FLAG antibody for ICAD (middle panel). DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.Ab247711 is the carrier-free version of ab108924. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was. tumor and its cleavage upon activation of apoptosis by anti-Fas antibody. A hamster monoclonal antibody (mAb) against mouse CAD was established by immunizing. DF7947 at 1/100 staining Rat testis tissue by IHC-P. ICAD / DFF is a downstream molecule of caspases, participating in nuclear DNA.
#Icad antibody download
Antibodies should not be exposed to prolonged high temperatures. Download scientific diagram No expression of CAD in ICAD-/- cells. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. ICAD Antibody, ICAD, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, Icad, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.Īntibody can be stored at 4☌ for three months and at -20☌ for up to one year. Rabbit polyclonal ICAD antibody was raised against a peptide corresponding to amino acids 312 to 331 of mouse ICAD.Īntibody is supplied in PBS containing 0.02% sodium azide.ĭuring shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial.
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